The Apolipoprotein A-I Polyclonal Antibody (CAB1129) is a powerful tool for researchers studying apolipoprotein A-I, a key component of high-density lipoprotein (HDL) particles that play a critical role in lipid metabolism and cardiovascular health. This antibody, raised in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot and immunohistochemistry applications.Apolipoprotein A-I is known for its role in reverse cholesterol transport, promoting the removal of excess cholesterol from peripheral tissues and transporting it back to the liver for excretion.
Dysregulation of apolipoprotein A-I has been linked to cardiovascular diseases such as atherosclerosis, making it a valuable target for therapeutic interventions.By targeting apolipoprotein A-I, researchers can gain insights into the mechanisms underlying lipid metabolism and cardiovascular health, paving the way for the development of novel treatment strategies for cardiovascular diseases. The Apolipoprotein A-I Polyclonal Antibody (CAB1129) is a valuable tool for unraveling the complexities of apolipoprotein A-I biology and its implications for human health.
Product Name:
APOA1 Rabbit Polyclonal Antibody
SKU:
CAB1129
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1).
Hep G2,Mouse plasma,Mouse liver,Rat liver,Rat plasma
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
28kDa
Observed MW:
29kDa
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Purification Method:
Affinity purification
Gene ID:
335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using APOA1 Rabbit pAb (CAB1129) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): HAP1Exposure time: 180s.
