The ApoA1 Polyclonal Antibody (CAB14211) is specifically designed for research involving Apolipoprotein A1 (ApoA1), a key component of high-density lipoprotein (HDL) known for its role in cholesterol metabolism and cardiovascular health. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.ApoA1 is a critical player in the process of reverse cholesterol transport, promoting the efflux of cholesterol from tissues to the liver for excretion. Dysregulation of ApoA1 has been associated with cardiovascular diseases, making it a promising target for therapeutic interventions aimed at improving lipid metabolism and reducing the risk of atherosclerosis.
Research involving the ApoA1 Polyclonal Antibody can provide valuable insights into the mechanisms underlying lipid metabolism and cardiovascular health. By studying the expression and function of ApoA1 in various cell types and disease states, researchers can better understand its role in maintaining lipid homeostasis and potentially identify new approaches for preventing and treating cardiovascular disorders.
Product Name:
APOA1 Rabbit Polyclonal Antibody
SKU:
CAB14211
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1).
Mouse lung,mouse liver,mouse small intestine,rat lung,rat liver
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
28kDa
Observed MW:
31kDa
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Purification Method:
Affinity purification
Gene ID:
335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using APOA1 Rabbit pAb (CAB14211) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
