The Anti-SAE1 Antibody (CAB9960) is a high-quality polyclonal antibody designed for research involving SAE1, a small ubiquitin-like modifier (SUMO)-activating enzyme. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal tool for studying the role of SAE1 in various cellular processes.SAE1 is a critical enzyme involved in the SUMOylation pathway, which regulates protein function and cellular signaling. Research has shown that dysregulation of the SUMOylation pathway can contribute to diseases such as cancer, neurodegenerative disorders, and viral infections.
By targeting SAE1 with this antibody, researchers can investigate its role in SUMOylation and its potential implications in disease development.Validated for use in Western blot applications, the Anti-SAE1 Antibody binds specifically to the SAE1 protein, allowing for accurate detection and analysis in different cell types. This antibody is essential for studies in molecular biology, biochemistry, and cellular signaling pathways, providing valuable insights into the mechanisms of SUMOylation and its impact on cellular function.
Product Name:
SAE1 Rabbit Polyclonal Antibody
SKU:
CAB9960
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1).
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
Purification Method:
Affinity purification
Gene ID:
10055
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SAE1 antibody (CAB9960) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
