The Anti-Phospho-Vav1 (Y174) Antibody (CABP0180) is a highly specific tool for research involving Vav1, a signaling protein that plays a key role in cell activation and immune responses. This antibody, generated in rabbits, is particularly efficient in detecting phosphorylation at Tyrosine 174 of the Vav1 protein in human samples. It has been validated for optimal performance in Western blot applications, allowing for precise analysis of Vav1 phosphorylation in a variety of cell types.Vav1 is a critical regulator of immune cell activation and function, influencing processes such as T-cell activation, B-cell development, and leukocyte migration. Phosphorylation of Vav1 at Y174 is known to enhance its activity, suggesting a potential role in signal transduction pathways related to immune response and inflammation.
Research involving this antibody can provide valuable insights into the molecular mechanisms underlying immune cell activation and may have implications for studies in immunology, cancer biology, and inflammatory diseases.By targeting the phosphorylation of Vav1 at Y174, this antibody offers a unique opportunity to investigate the signaling events that govern immune cell function and communication. Understanding the regulation of Vav1 phosphorylation could lead to the development of novel therapeutic strategies for manipulating immune responses in various disease contexts.
Product Name:
Phospho-VAV1-Y174 Rabbit Polyclonal Antibody
SKU:
CABP0180
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y174 of human VAV1
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
7409
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (CABP0180).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
