The Anti-Phospho-Vangl2 (S79/S82/S84) Antibody (CABP1207) is a valuable tool for researchers studying Vangl2, a protein involved in cell polarity and tissue patterning. This antibody, produced in rabbits, is highly specific for phosphorylated forms of Vangl2 at serine 79, 82, and 84, making it ideal for detecting and analyzing these post-translational modifications in various cell types.Vangl2 is a key player in signaling pathways that regulate cell migration and tissue organization, making it a crucial protein in embryonic development and disease progression.
The phosphorylation of specific residues on Vangl2 can impact its function and localization within the cell, influencing its role in cellular processes. By using the Anti-Phospho-Vangl2 (S79/S82/S84) Antibody, researchers can gain insights into the molecular mechanisms underlying these processes and potentially uncover new therapeutic targets for conditions such as cancer and developmental disorders.
The protein encoded by this gene is a membrane protein involved in the regulation of planar cell polarity, especially in the stereociliary bundles of the cochlea. The encoded protein transmits directional signals to individual cells or groups of cells in epithelial sheets. This protein is also involved in the development of the neural plate.
Purification Method:
Affinity purification
Gene ID:
57216
Clone Number:
ARC5022
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-VANGL2-S79/S82/S84 Rabbit mAb antibody (CABP1207) at1:1000 dilution.HeLa and C6 cells were treated by wnt(100ng/ml) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
