The Anti-Phospho-PLN (S16/T17) Antibody (CABP0956) is a valuable tool for researchers studying the phosphorylation of Phospholamban (PLN) at serine 16 and threonine 17 sites. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.PLN is a key regulator of cardiac muscle contractility, with its phosphorylation at the S16/T17 sites playing a critical role in modulating its activity. Dysregulation of PLN phosphorylation has been implicated in various cardiac disorders, making it an important target for cardiovascular research.
By specifically binding to the phosphorylated form of PLN at the S16/T17 sites, this antibody enables accurate detection and quantification of phosphorylated PLN in cells and tissues. Its application in research related to cardiac function, heart failure, and other cardiovascular diseases makes it an essential tool for scientists seeking to better understand the molecular mechanisms underlying these conditions.
Product Name:
Phospho-PLN-S16/T17 Rabbit Polyclonal Antibody
SKU:
CABP0956
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S16 & T17 of human PLN (NP_002658.1).
Sequence:
ASTI E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
PLB; CMD1P; CMH18; Phospho-PLN-S16/T17
Positive Sample:
HeLa,C6
Conjugate:
Unconjugated
Cellular Localization:
endoplasmic reticulum, mitochondrial membrane, mitochondrion, perinuclear region of cytoplasm
Calculated MW:
6kDa
Observed MW:
13kDa/17kDa
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.
Purification Method:
Affinity purification
Gene ID:
5350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit pAb (CABP0956) at 1:1000 dilution.Hela cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
