The Anti-Phospho-MAPK14 (T180/Y182) + MAPK11 (T180/Y182) + MAPK13 (T180/Y182) + MAPK12 (T183/Y1845) Antibody (CABP1256) is a highly specific and reliable tool for studying the phosphorylation of MAPK proteins in various biological processes. This polyclonal antibody, produced in rabbits, has been validated for use in Western blot applications and shows high reactivity with human samples.MAPK proteins are key regulators of cellular signaling pathways, playing crucial roles in cell proliferation, differentiation, and survival. The phosphorylation of specific residues, such as T180/Y182 in MAPK14 (p38), T180/Y182 in MAPK11 (p38β), T180/Y182 in MAPK13 (p38γ), and T183/Y1845 in MAPK12 (p38δ), is known to influence their activity and downstream signaling cascades.
The CABP1256 antibody specifically targets these phosphorylation sites, allowing for detailed analysis of MAPK activation in response to different stimuli.Research in the fields of immunology, cancer biology, and inflammation can greatly benefit from the use of this antibody to investigate the role of MAPK signaling pathways in disease pathogenesis. By understanding the phosphorylation status of MAPK proteins, researchers can gain insights into the molecular mechanisms underlying various diseases and potentially identify novel therapeutic targets for intervention. Overall, the CAB
A synthetic phosphorylated peptide around T180 & Y182 of human MAPK14MAPK11 (NP_002742.3).
Sequence:
MTGY V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Positive Sample:
C6
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, nucleoplasm, nucleus
Observed MW:
43kDa
Mapk14 (phosphoT180/Y182) monoclonal antibody is provided, clone number M139 is a monoclonal antibody prepared from mice, its clone number is M139. Mapk14 is also known as CSBP, CSPB1, Csbp1, Csbp2, Exip, Hog, MGC105413, Mxi2, Prkm14, Prkm15, RK, Sapk2A, p38, p38Hog, p38alpha; The full name of its gene is Mitogen Activated Protein Kinase 14.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 antibody (CABP1256) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
