The Anti-phospho-IKBAlpha (S36) Antibody (CABP1069) is a novel tool for investigating the phosphorylation of IKBAlpha at Serine 36, a key regulatory event in the NF-κB signaling pathway. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blotting applications.IKBAlpha is a crucial component of the NF-κB pathway, which plays a central role in regulating inflammation, immune response, and cell survival. Phosphorylation of IKBAlpha at Serine 36 is known to trigger its degradation, leading to the activation of NF-κB transcription factors and the subsequent expression of inflammatory genes.
The Anti-phospho-IKBAlpha (S36) Antibody enables researchers to detect and analyze the phosphorylation status of IKBAlpha in various cell types, providing valuable insights into the molecular mechanisms underlying inflammatory diseases, cancer, and other pathological conditions. By understanding the regulation of IKBAlpha phosphorylation, researchers can develop targeted therapies aimed at modulating NF-κB signaling for the treatment of these diseases.
Product Name:
Phospho-IκBα-S36 Rabbit Polyclonal Antibody
SKU:
CABP1069
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S36 of human IκBα (NP_065390.1).
Sequence:
LDSM K
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S36
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa
Observed MW:
45kDa
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Purification Method:
Affinity purification
Gene ID:
4792
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-IκBα-S36 pAb (CABP1069) at 1:1000 dilution or IKB alpha antibody (CAB11397).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
