The Anti-PCNT Antibody (CAB20161) is a polyclonal antibody designed for research involving pericentrin (PCNT), a protein known for its critical role in centrosome organization and cell division. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in various applications, including immunofluorescence, immunohistochemistry, and Western blot.Pericentrin is a key player in centrosome function, having implications in cell cycle progression, microtubule organization, and cell division accuracy. Dysregulation of PCNT has been linked to various human diseases, including cancer and neurological disorders.
Therefore, studying PCNT expression and localization is vital for understanding its role in cellular processes and disease pathogenesis.The Anti-PCNT Antibody binds specifically to PCNT, allowing for accurate detection and visualization of PCNT within cells, tissues, or protein lysates. Its high specificity and sensitivity make it an ideal tool for researchers studying centrosome biology, cell division, and diseases associated with centrosome dysfunction. With its applications in various research techniques, this antibody is a valuable asset for studies in cell biology, oncology, and neurology.
Product Name:
PCNT Rabbit Polyclonal Antibody
SKU:
CAB20161
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 95-212 of human PCNT (NP_006022.3).
The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5116
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PCNT Rabbit pAb (CAB20161) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
