Anti-Mouse MHC Class I (H-2Kb) [AF6-88.5] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse MHC Class I [H-2Kb] AF6-88.
5 In Vivo Antibody - Low Endotoxin from Assay Genie, a precision-engineered monoclonal antibody tailored for in vivo applications. This antibody is specific to the MHC Class I H-2Kb molecule, a pivotal component in antigen presentation, making it indispensable for research in immunology, transplant biology, and related fields. With a mouse IgG2b isotype, it guarantees exceptional purity and low endotoxin levels (<1.0 EU/mg), making it suitable for ELISA, flow cytometry, immunohistochemistry, and other assays. The antibody is formulated in phosphate-buffered saline to ensure stability and effectiveness, and is available in multiple sizes to cater to varied research needs. MHC Class I molecules are critical for the presentation of intracellular antigenic peptides to CD8+ T cells, thus playing an essential role in the immune surveillance against pathogens and tumors. The H-2Kb protein is a specific type of mouse MHC Class I molecule that is extensively studied in immunological research. Enhance your research capability with this dependable and versatile antibody, optimized for various experimental applications.
Product Name:
Anti-Mouse MHC Class I (H-2Kb) (Clone AF6-88.5) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0303
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
AF6-88.5
Protein:
MHC Class I
Product Type:
Monoclonal Antibody
Synonyms:
H-2Kb
Isotype:
IgG2a k
Reactivity:
Mouse
Applications:
FA, FC, IHC FF, In Vivo, IP
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FA, FC, IHC FF, In Vivo, IP
Reactivity:
Mouse
Host Species:
Mouse
Specificity:
Clone AF6-88.5, Anti-Mouse H-2Kb, recognizes an epitope on mouse MHC class I H-2K haplotype b. AF6-88.5 does not cross-react with other H-2K haplotypes, including d, f, j, k, p, q, r, s, u, v.
Antigen Distribution:
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen and is ubiquitously expressed on nucleated cells from mice of the H-2Kb haplotype, including C57BL/6. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.
Immunogen:
Mouse C57BL Splenocytes
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
H-2Kb antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype b (H-2Kb). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kb plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I to augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.
![Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214344/569467/anti-mouse-h-2kb-mhc-class-i-y-3-in-vivo-antibody-low-endotoxin__11576.1673496996.jpg?c=2 "Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low Endotoxin")
![Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214345/569421/anti-mouse-h-2kb-mhc-class-i-y-3-in-vivo-antibody-ultra-low-endotoxin__09050.1673496870.jpg?c=2 "Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin")
