The Anti-Lactic Acid Histone H3K14 Polyclonal Antibody (CAB18808) is a valuable tool for research involving histone modification, specifically targeting the histone H3K14 site. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in applications such as Western blotting and immunohistochemistry.Histone H3K14 acetylation is a key epigenetic modification associated with gene expression regulation, DNA repair, and chromatin remodeling. Dysregulation of this modification has been linked to various diseases, including cancer and neurological disorders.
By targeting the H3K14 acetylation site, the antibody enables researchers to investigate the role of histone modifications in these disease processes and potential therapeutic interventions.With its high specificity and sensitivity, the Anti-Lactic Acid Histone H3K14 Polyclonal Antibody (CAB18808) is a valuable tool for studying epigenetic regulation and its impact on cellular function. Its applications in research on cancer, neurobiology, and other fields make it a valuable asset for scientists seeking to understand the role of histone modifications in health and disease.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Lactic acid-Histone H3-K14 Rabbit pAb (CAB18808) at 1:1000 dilution.MCF7 cells and NIH/3T3 cells and C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
