Based on the information from the URL provided, here is a product description:The Anti-Human IgM Antibody (CAB8677) is a highly specific and sensitive tool for research involving IgM, an important component of the human immune system. This polyclonal antibody, generated in rabbits, has been rigorously validated for use in various immunoassays, including Western blot and immunofluorescence applications. It binds specifically to the IgM protein, allowing for precise detection and analysis in a wide range of samples and cell types.
IgM is the first-line defense antibody produced by the body in response to infections, making it a critical marker for studying immune responses and diseases. Its detection and quantification are essential for monitoring immune function, evaluating disease progression, and developing targeted therapies. The Anti-Human IgM Antibody (CAB8677) provides researchers with a reliable tool to advance their studies in immunology, infectious diseases, and autoimmune disorders.
Product Name:
Human IgM Rabbit Polyclonal Antibody
SKU:
CAB8677
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 213-453 of human IGHM (P01871).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
49kDa
Observed MW:
80kDa
Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).
Purification Method:
Affinity purification
Gene ID:
3507
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using Human IgM Rabbit pAb (CAB8677) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
