The Anti-Dysferlin Romeo Antibody (CAB19572) is a high-quality research tool designed for studying dysferlin, a protein involved in muscle membrane repair. This polyclonal antibody, raised in rabbits, shows strong reactivity with dysferlin in human samples and has been validated for use in Western blot applications. It specifically binds to dysferlin, allowing for accurate detection and analysis in various cell types.Dysferlin is a key player in muscle regeneration and maintenance, making it a focus of research in muscular dystrophies and neuromuscular disorders.
Mutations in the dysferlin gene can lead to impaired muscle membrane repair and muscle wasting, highlighting the importance of studying dysferlin function and regulation. The Anti-Dysferlin Romeo Antibody provides researchers with a reliable tool for investigating dysferlin-related mechanisms and developing potential therapies for muscle diseases.
Product Name:
Dysferlin (Romeo) Rabbit Monoclonal Antibody
SKU:
CAB19572
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Dysferlin (Romeo) (O75923).
The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8291
Clone Number:
ARC2178
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from RD cells, using Dysferlin (Romeo) Rabbit mAb (CAB19572) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
