The Anti-Dimethyl Histone H3 (K79) Antibody (CAB20822) is a key tool for researchers studying histone modifications, specifically focusing on dimethylation of histone H3 at lysine 79. This antibody, raised in rabbits, is highly specific for this modification and is validated for use in various applications including Western blot, immunoprecipitation, and immunofluorescence.Histone modifications, such as dimethylation of histone H3 at K79, play a crucial role in gene regulation and chromatin structure, impacting diverse biological processes including transcriptional activation, DNA repair, and cell cycle regulation.
The Anti-Dimethyl Histone H3 (K79) Antibody enables researchers to investigate the role of this specific histone modification in various cellular processes, making it a valuable tool for epigenetics and chromatin biology research.Understanding the function and regulation of histone modifications like dimethylation of histone H3 at K79 is essential for unraveling the complexity of gene expression and epigenetic regulation. This antibody, with its high specificity and sensitivity, provides researchers with a reliable tool to advance their studies in the field of epigenetics and chromatin biology.
Product Name:
DiMethyl-Histone H3-K79 Polyclonal Antibody
SKU:
CAB20822
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic dimethylated peptide around K79 of human histone H3 (NP_003520.1).
Sequence:
QDFK T
Tested Applications:
WBChIPELISA
Recommended Dilution:
WB,1:100 - 1:500 ChIP,5μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using DiMethyl-Histone H3-K79 Rabbit pAb (CAB20822) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
