The ALPL Polyclonal Antibody (CAB12396) is a valuable tool for researchers studying the ALPL protein, also known as alkaline phosphatase. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to ALPL, this antibody enables detection and analysis of the enzyme in various cell types, making it ideal for research in areas such as bone metabolism, liver function, and developmental biology.ALPL is a critical enzyme involved in bone mineralization and plays a key role in the regulation of various cellular processes. Dysregulation of ALPL has been implicated in a variety of diseases, including metabolic bone disorders, liver diseases, and certain cancers.
By studying the expression and activity of ALPL using this antibody, researchers can gain important insights into the underlying mechanisms of these conditions and develop potential therapeutic strategies.Overall, the ALPL Polyclonal Antibody (CAB12396) offers a reliable and specific tool for exploring the function and significance of the ALPL enzyme in both normal and diseased states. Its high reactivity and specificity make it a valuable asset for researchers seeking to advance our understanding of ALPL biology and its potential implications for human health.
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
Purification Method:
Affinity purification
Gene ID:
249
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Alkaline Phosphatase (ALPL) Rabbit pAb (CAB12396) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
