The ALG10 Polyclonal Antibody (PACO60068) is a valuable tool for researchers studying ALG10, an enzyme involved in protein glycosylation processes. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ALG10 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it suitable for investigations in glycobiology and related fields.ALG10 is essential for the proper glycosylation of proteins, a crucial process that impacts protein structure and function.
Dysregulation of protein glycosylation has been linked to various diseases, including cancer and genetic disorders. Therefore, studying ALG10 and its role in glycosylation pathways is important for uncovering potential therapeutic targets and understanding disease mechanisms. The ALG10 Polyclonal Antibody opens up opportunities for in-depth research into these areas, advancing our knowledge of protein glycosylation and its implications in health and disease.
Antibody Name:
ALG10 Antibody (PACO60068)
Antibody SKU:
PACO60068
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase protein (197-249AA)
IHC image of PACO60068 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol.
ALG10: Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol. Belongs to the ALG10 glucosyltransferase family.Protein type: Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.256; Membrane protein, integral; Membrane protein, multi-pass; TransferaseChromosomal Location of Human Ortholog: 12p11.1Disease: Long Qt Syndrome 2
UniProt Protein Details:
NCBI Summary:
This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010]
