The ALDOB Polyclonal Antibody (CAB3728) is a highly specific and sensitive tool designed for research involving ALDOB, a key enzyme in fructose metabolism. This antibody, generated in rabbits, has been rigorously validated for use in various applications including Western blot and immunohistochemistry.ALDOB, or aldolase B, plays a crucial role in catalyzing the conversion of fructose-1-phosphate to glyceraldehyde and dihydroxyacetone phosphate. Dysregulation of ALDOB has been implicated in various metabolic disorders such as hereditary fructose intolerance and liver diseases.
Understanding the expression and function of ALDOB is essential for studying these conditions and developing potential therapeutic interventions.With its high reactivity and specificity for human ALDOB, the CAB3728 antibody offers researchers a reliable tool for detecting and quantifying ALDOB protein levels in different cell and tissue types. Its versatility makes it an invaluable resource for studies focusing on metabolic diseases, liver function, and fructose metabolism.
Product Name:
ALDOB Rabbit Polyclonal Antibody
SKU:
CAB3728
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALDOB (NP_000026.2).
Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.
Purification Method:
Affinity purification
Gene ID:
229
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALDOB antibody (CAB3728) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
