The ADCY5 Polyclonal Antibody (PAC05861) is a versatile tool for researchers studying ADCY5, a key enzyme involved in intracellular signaling pathways. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blotting. By binding to the ADCY5 protein, this antibody allows for precise detection and analysis in a wide range of cell types, making it a valuable asset for studies in cell signaling and drug development.ADCY5 is an important regulator of cellular function, particularly in processes related to neurotransmission and hormone secretion. Dysregulation of ADCY5 has been implicated in various diseases, including neurological disorders and endocrine disorders.
Investigating the role of ADCY5 can provide valuable insights into the pathogenesis of these conditions and may lead to the development of novel therapeutic strategies targeting this enzyme.Overall, the ADCY5 Polyclonal Antibody is a reliable tool for researchers interested in exploring the functions of ADCY5 and its implications in health and disease. Its high specificity and sensitivity make it an invaluable resource for studies aiming to elucidate the mechanisms underlying ADCY5-mediated signaling pathways and identify potential therapeutic targets.
Antibody Name:
ADCY5 Antibody (PACO58681)
Antibody SKU:
PACO58681
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Adenylate cyclase type 5 protein (584-719AA)
Immunofluorescence staining of Hela cells with PACO58681 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Mediates signaling downstream of ADRB1. Regulates the increase of free cytosolic Ca(2+) in response to increased blood glucose levels and contributes to the regulation of Ca(2+)-dependent insulin secretion.
Synonyms:
Adenylate cyclase type 5 (EC 4.6.1.1) (ATP pyrophosphate-lyase 5) (Adenylate cyclase type V) (Adenylyl cyclase 5) (AC5), ADCY5
UniProt Protein Function:
ADCY5: This is a membrane-bound, calcium-inhibitable adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Adenylyl cyclase; Nucleotide Metabolism - purine; Transporter, aquaporin family; EC 4.6.1.1; Transporter; Membrane protein, integral; LyaseChromosomal Location of Human Ortholog: 3q21.1Cellular Component: integral to membrane; plasma membraneMolecular Function: adenylate cyclase activity; adenylate cyclase binding; protein heterodimerization activityBiological Process: activation of protein kinase A; adenylate cyclase activation; cAMP biosynthetic process; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, adenylate cyclase inhibiting pathway; renal water homeostasisDisease: Dyskinesia, Familial, With Facial Myokymia
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
