The ADAT1 Antibody (PAC040486) is a valuable tool for researchers studying ADAT1, a crucial enzyme involved in RNA editing and modification processes. This polyclonal antibody is raised in rabbits and exhibits high reactivity with human samples, making it an ideal choice for Western blot applications. By specifically binding to the ADAT1 protein, this antibody allows for precise detection and analysis in a variety of cell types.ADAT1 is known for its role in catalyzing the deamination of adenosine to inosine in tRNA molecules, which is essential for maintaining proper protein translation and cellular function.
Dysregulation of ADAT1 has been implicated in various diseases, including cancer and neurological disorders, highlighting the importance of studying its function and activity. By targeting ADAT1 with this antibody, researchers can gain valuable insights into RNA editing mechanisms and potentially develop novel therapeutic strategies for diseases linked to ADAT1 dysfunction.
Western blot. All lanes: ADAT1 antibody at 6µg/ml + MDA-MB-231 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 56, 39 kDa. Observed band size: 56 kDa.
Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO40486 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human skin tissue using PACO40486 at dilution of 1:100.
Background:
Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
ADAT1: Specifically deaminates adenosine-37 to inosine in tRNA- Ala. Belongs to the ADAT1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: RNA processing; EC 3.5.4.34; HydrolaseChromosomal Location of Human Ortholog: 16q23.1Molecular Function: RNA binding; tRNA-specific adenosine deaminase activityBiological Process: tRNA processing
UniProt Protein Details:
NCBI Summary:
This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]
