The ACSL4 Polyclonal Antibody (CAB16848) is a valuable tool for researchers studying ACSL4, a key enzyme involved in lipid metabolism and cellular signaling pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.ACSL4, also known as long-chain-fatty-acid-CoA ligase 4, plays a crucial role in lipid metabolism by catalyzing the conversion of long-chain fatty acids to their respective acyl-CoA derivatives. Dysregulation of ACSL4 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.
By specifically binding to the ACSL4 protein, this polyclonal antibody enables researchers to detect and analyze ACSL4 levels in different cell types, providing valuable insights into the role of this enzyme in cellular function and disease pathology. Its versatility and reliability make it a valuable tool for investigations in fields such as lipid biology, cancer research, and metabolic disorders.
Product Name:
ACSL4 Polyclonal Antibody
SKU:
CAB16848
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACSL4 (NP_004449.1).
Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2182
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of HepG2, using ACSL4 antibody (CAB16848) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
