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ACOT1 Antibody (PACO56406)

SKU:
PACO56406
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • ACOT1 Antibody PACO56406
  • ACOT1 Antibody PACO56406
€339
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Description

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ACOT1 Antibody (PACO56406)

The ACOT1 Antibody (PAC056406) is a polyclonal antibody designed for research involving ACOT1, an enzyme involved in lipid metabolism. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunofluorescence. ACOT1 plays a critical role in the hydrolysis of acyl-CoA esters, making it essential for fatty acid metabolism. Its dysregulation has been linked to various metabolic disorders, making it a valuable target for research in areas such as obesity, diabetes, and cardiovascular disease.

By using the ACOT1 Antibody, researchers can detect and analyze the expression of ACOT1 in different cell types and tissues, aiding in the understanding of its functions and potential implications in disease. This antibody is a valuable tool for studies in lipid metabolism, metabolic diseases, and drug discovery efforts aimed at targeting ACOT1 for therapeutic purposes.

Antibody Name:ACOT1 Antibody (PACO56406)
Antibody SKU:PACO56406
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human Acyl-coenzyme A thioesterase 1 protein (258-350AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageIHC image of PACO56406 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageImmunofluorescence staining of Hela cells with PACO56406 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid, and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acid, and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity).
Synonyms:Acyl-coenzyme A thioesterase 1 (Acyl-CoA thioesterase 1) (EC 3.1.2.2) (CTE-I) (CTE-Ib) (Inducible cytosolic acyl-coenzyme A thioester hydrolase) (Long chain acyl-CoA thioester hydrolase) (Long chain acyl-CoA hydrolase), ACOT1, CTE1
UniProt Protein Function:ACOT1: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16. Belongs to the C/M/P thioester hydrolase family.Protein type: EC 3.1.2.2; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesisChromosomal Location of Human Ortholog: 14q24.3Cellular Component: cytosolMolecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activityBiological Process: acyl-CoA metabolic process; very-long-chain fatty acid metabolic process; long-chain fatty acid metabolic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q86TX2
NCBI GenInfo Identifier:81230485
NCBI Gene ID:641371
NCBI Accession:NP_001032238.1
UniProt Secondary Accession:Q86TX2,Q3I5F9, A1L173
UniProt Related Accession:Q86TX2
Molecular Weight:46,277 Da
NCBI Full Name:acyl-coenzyme A thioesterase 1
NCBI Synonym Full Names:acyl-CoA thioesterase 1
NCBI Official Symbol:ACOT1  
NCBI Official Synonym Symbols:ACH2; CTE-1; LACH2  
NCBI Protein Information:acyl-coenzyme A thioesterase 1; CTE-I; CTE-Ib; long chain acyl-CoA hydrolase; long chain acyl-CoA thioester hydrolase; inducible cytosolic acyl-coenzyme A thioester hydrolase
UniProt Protein Name:Acyl-coenzyme A thioesterase 1
UniProt Synonym Protein Names:CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase
Protein Family:Acyl-coenzyme A thioesterase
UniProt Gene Name:ACOT1  
UniProt Entry Name:ACOT1_HUMAN
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Secondary Antibody
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