The Acetyl-FOXO1 (K262/K265/K274) Polyclonal Antibody (CAB17406) is a valuable tool for research involving FOXO1, a transcription factor that plays a key role in regulating cell growth, differentiation, and metabolism. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for use in Western blot applications. By targeting the acetylated form of FOXO1 at residues K262, K265, and K274, this antibody enables researchers to study the regulation of FOXO1 activity in various cellular processes.FOXO1, a member of the FOXO subfamily of forkhead transcription factors, is known to be acetylated at multiple lysine residues, including K262, K265, and K274, which can impact its transcriptional activity and subcellular localization.
Understanding the acetylation status of FOXO1 is important for deciphering its role in cellular functions such as apoptosis, cell cycle regulation, and oxidative stress response. This antibody provides a powerful tool for investigating the molecular mechanisms underlying FOXO1 acetylation and its implications in physiological and pathological conditions. Overall, the Acetyl-FOXO1 (K262/K265/K274) Polyclonal Antibody (CAB17406) offers researchers a reliable reagent for studying the role of FOXO1 acetylation in various biological processes, with potential applications in cancer research, metabolic disorders, and aging
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
Purification Method:
Affinity purification
Gene ID:
2308
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HCT 116 cells using Acetyl-FOXO1-K262/K265/K274 Rabbit pAb (CAB17406) at 1:400 dilution. HCT 116 cells were treated by H2O2(0.5mM) at 37℃ for 12 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
