The Acetyl Alpha Tubulin (K40) Polyclonal Antibody is a valuable tool for researchers studying tubulin acetylation, a key post-translational modification involved in microtubule stability and cell motility. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards detecting acetylated alpha tubulin in various biological samples, including human tissues and cell lines.Acetylation of alpha tubulin at lysine 40 plays a crucial role in diverse cellular processes such as cell division, intracellular transport, and signal transduction. Dysregulation of tubulin acetylation has been implicated in various diseases, including neurodegenerative disorders and cancer.
By targeting the acetylated form of alpha tubulin, researchers can gain insights into the molecular mechanisms underlying these pathological conditions.The Acetyl Alpha Tubulin (K40) Polyclonal Antibody is validated for use in Western blotting, immunofluorescence, and immunohistochemistry applications, enabling researchers to visualize and quantify acetylated alpha tubulin in different experimental setups. Its versatility and reliability make it an indispensable tool for investigations in cell biology, neuroscience, and oncology, paving the way for innovative discoveries and potential therapeutic interventions.
Product Name:
Acetyl-alpha Tubulin -K40 Polyclonal Antibody
SKU:
CAB23069
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic acetylated peptide around K40 of human alpha Tubulin.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
LIS3; TUBA3; B-ALPHA-1; Acetyl-alpha Tubulin -K40
Positive Sample:
HeLa,Mouse testis,Rat testis
Conjugate:
Unconjugated
Calculated MW:
50kDa
Observed MW:
50kDa
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
7846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa, using Acetyl-alpha Tubulin -K40 antibody (CAB23069) at 1:500 dilution.HeLa cells were treated by Sodium butyrate (5 mM) for 16 hours after serum-starvation 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
