The ZP3 Polyclonal Antibody (CAB13156) is specially crafted for research on the ZP3 protein, an essential component of the zona pellucida surrounding oocytes. This antibody, produced in rabbits, displays high reactivity towards human samples and is validated for use in Western blotting applications. By binding to the ZP3 protein, researchers can effectively detect and analyze this key molecule in various cell types, making it a valuable tool for studies in reproductive biology and fertility research.ZP3, a critical player in sperm-egg interaction and fertilization, is crucial for successful reproduction in mammals.
Understanding the function and regulation of ZP3 is essential for advancements in assisted reproductive technologies and contraception methods. By studying the role of ZP3 in fertility processes, researchers can gain insights into infertility disorders and potential targets for therapeutic interventions.Overall, the ZP3 Polyclonal Antibody (CAB13156) offers researchers a reliable tool to investigate the role of ZP3 in reproductive biology, opening up new possibilities for understanding and enhancing fertility outcomes.
Product Name:
ZP3 Rabbit Polyclonal Antibody
SKU:
CAB13156
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 246-345 of human ZP3 (NP_001103824.1).
Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space.
Calculated MW:
47kDa
Observed MW:
47kDa
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.
Purification Method:
Affinity purification
Gene ID:
7784
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using ZP3 Rabbit pAb (CAB13156) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
