The PSME3 Polyclonal Antibody (CAB12697) is specifically designed for research involving PSME3, a key regulatory protein involved in the activation of the immunoproteasome. The antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PSME3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent tool for studies in immunology and cancer research.PSME3, also known as Proteasome Activator Complex subunit 3, is essential for the function of the immunoproteasome, a specialized form of the proteasome that plays a crucial role in regulating the immune response. Dysregulation of PSME3 has been implicated in various diseases, including cancer and autoimmune disorders.
Thus, investigating the role of PSME3 can provide valuable insights into the underlying mechanisms of these conditions and potentially lead to the development of novel therapeutic strategies targeting the immunoproteasome pathway.In summary, the PSME3 Polyclonal Antibody (CAB12697) is a highly specific and reliable tool for studying the function and regulation of PSME3 in various biological contexts. Its application in Western blot analysis allows for accurate detection of PSME3 levels, making it an invaluable asset for researchers investigating the immunoproteasome and its implications in disease pathogenesis.
Product Name:
PSME3 Rabbit Polyclonal Antibody
SKU:
CAB12697
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-254 of human PSME3 (NP_005780.2).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSME3 Rabbit pAb (CAB12697) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
