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Porcine Interferon Beta (IFNb) ELISA Kit

SKU:
PRDL00073
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q29412
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IFNB1,IFN-B,IFB,IFF,IFNB,Interferon Beta 1 Fibroblast
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Porcine Interferon Beta (IFNb) ELISA Kit

The Porcine Interferon Beta (IFN-β) ELISA Kit is a reliable and accurate tool for the detection of IFN-β levels in porcine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Interferon Beta is a key cytokine involved in the immune response, particularly in the defense against viral infections and the regulation of inflammation.

Its levels can be indicative of the immune status of an individual, making it a valuable biomarker in infectious diseases, autoimmune disorders, and inflammatory conditions.With the Porcine Interferon Beta (IFN-β) ELISA Kit, researchers can confidently measure and analyze IFN-β levels in porcine samples, providing valuable insights into immune responses and potential therapeutic targets.

Product Name:Porcine Interferon Beta (IFNb) ELISA Kit
Product Code:PRDL00073
Size:96 Assays
Target:IFNb
Synonyms:IFNB1, IFN-B, IFB, IFF, IFNB, Interferon Beta 1 Fibroblast
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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