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Human Phospholipase A2, Group X (PLA2G10) ELISA Kit

SKU:
HUDL02316
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O15496
Sensitivity:
37pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
GXPLA2,GX sPLA2,sPLA2-X,Group 10 secretory phospholipase A2,Phosphatidylcholine 2-acylhydrolase 10
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Phospholipase A2, Group X (PLA2G10) ELISA Kit

The Human Phospholipase A2 Group X (PLA2G10) ELISA Kit is a reliable tool for the accurate measurement of PLA2G10 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PLA2G10 is an important enzyme involved in lipid metabolism and inflammatory processes. It plays a key role in various diseases such as inflammation, atherosclerosis, and cancer.

By detecting PLA2G10 levels, researchers can gain valuable insights into the pathophysiology of these conditions and develop potential therapeutic strategies.With its user-friendly format and high performance, the Human Phospholipase A2 Group X (PLA2G10) ELISA Kit is a valuable tool for studying the role of PLA2G10 in human health and disease. Get reliable and accurate results with this innovative ELISA kit from Assay Genie.

Product Name:Human Phospholipase A2, Group X (PLA2G10) ELISA Kit
Product Code:HUDL02316
Size:96 Assays
Target:PLA2G10
Synonyms:GXPLA2, GX sPLA2, sPLA2-X, Group 10 secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 10
Detection Method:Sandwich
Range:78.1-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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