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Human Interleukin 18 (IL-18) ELISA Kit

SKU:
HUDL01478
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q14116
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IGIF,IL-1g,IL1F4,IL1-F4,Interferon-Gamma-Inducing Factor,Interleukin-1 Family Member 4,Iboctadekin,Interleukin-1 Gamma
Reactivity:
Human
Sample Type:
Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 18 (IL-18) ELISA Kit

The Human Interleukin 18 (IL-18) ELISA Kit from Assay Genie is a reliable tool for the accurate measurement of IL-18 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.IL-18 is a key cytokine involved in regulating immune responses and inflammation. Abnormal levels of IL-18 have been associated with various diseases, including inflammatory disorders, autoimmune diseases, and infectious diseases.

By measuring IL-18 levels using this ELISA kit, researchers can gain valuable insights into the role of IL-18 in disease pathogenesis and potentially identify novel therapeutic targets.Overall, the Human Interleukin 18 (IL-18) ELISA Kit from Assay Genie is a valuable tool for researchers studying the role of IL-18 in health and disease, offering high-quality performance and reliable results for scientific investigations.

Product Name:Human Interleukin 18 (IL-18) ELISA Kit
Product Code:HUDL01478
Size:96 Assays
Target:IL18
Synonyms:IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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