Human GDNF Recombinant Protein (RPPB0295)
- SKU:
- RPPB0295
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P39905
- Research Area:
- Neurotrophins
Description
| Product Name: | Human GDNF Recombinant Protein |
| Product Code: | RPPB0295 |
| Size: | 10µg |
| Species: | Human |
| Target: | GDNF |
| Synonyms: | ATF1, ATF2, HFB1-GDNF, GDNF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | GDNF was lyophilized from a 0.2�m filtered concentrated solution in 1×PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI |
| Biological Activity: | The ED50 was determined by the proliferation of rat C6 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0x107 units/mg. |
GDNF promotes the survival and differentiation of minergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.GDNF enhances survival and morphological differentiation of minergic neurons and increases their high-affinity uptake.
Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a non-glycosylated disulfide-linked homodimer containing 2 x 135 amino acids and having a total molecular mass of approximately 30kDa. GDNF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 5p13.1-p12 Cellular Component: extracellular region Molecular Function:protein homodimerization activity; growth factor activity; receptor binding Biological Process: positive regulation of dopamine secretion; axon guidance; nervous system development; peristalsis; adult locomotory behavior; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; signal transduction; enteric nervous system development; sympathetic nervous system development; ureteric bud branching; regulation of gene expression; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; positive regulation of cell differentiation; postsynaptic membrane organization; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma |
| NCBI Summary: | This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P39905 |
| NCBI GenInfo Identifier: | 729567 |
| NCBI Gene ID: | 2668 |
| NCBI Accession: | P39905.1 |
| UniProt Secondary Accession: | P39905,O95448, O95449, O95986, Q6FH33, Q96L44, Q9UD32 Q9UD33, Q9UMV2, Q9UP67, Q9UP97, B7WPK7, |
| UniProt Related Accession: | P39905,AAB33493,AAB33494 |
| Molecular Weight: | 18,123 Da |
| NCBI Full Name: | Glial cell line-derived neurotrophic factor |
| NCBI Synonym Full Names: | glial cell derived neurotrophic factor |
| NCBI Official Symbol: | GDNF�� |
| NCBI Official Synonym Symbols: | ATF1; ATF2; HSCR3; HFB1-GDNF�� |
| NCBI Protein Information: | glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor |
| UniProt Protein Name: | Glial cell line-derived neurotrophic factor |
| UniProt Synonym Protein Names: | Astrocyte-derived trophic factor; ATF |
| Protein Family: | GDNF family receptor |
| UniProt Gene Name: | GDNF�� |
| UniProt Entry Name: | GDNF_HUMAN |
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