Human DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) ELISA Kit
The Human DNAJ (Hsp40) Homolog, Subfamily C, Member 12 (DNAJC12) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of DNAJC12 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results for a variety of research applications.DNAJC12 is a member of the Hsp40 family of proteins, playing a crucial role in protein folding and unfolding processes within the cell.
Dysregulation of DNAJC12 has been linked to various diseases, including neurodegenerative disorders and cancer. By measuring DNAJC12 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human DNAJC12 ELISA Kit provides a reliable and efficient method for studying the role of DNAJC12 in health and disease, making it a valuable tool for researchers in the fields of molecular biology, biochemistry, and medicine.
Product Name:
Human DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) ELISA Kit
Product Code:
HUDL00848
Size:
96 Assays
Target:
DNAJC12
Synonyms:
JDP1, J Domain Protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
