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Human A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit

SKU:
HUDL00068
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UNA0
Sensitivity:
0.132ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ADMP-2,Aggrecanase-2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit

The Human ADAMTS5 ELISA Kit is a powerful tool for researchers looking to accurately measure levels of A Disintegrin and Metalloproteinase with Thrombospondin-5 (ADAMTS5) in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for reliable and reproducible results across a variety of research applications.ADAMTS5 is a key enzyme involved in extracellular matrix remodeling and has been implicated in various pathological conditions, including osteoarthritis and cardiovascular diseases.

By accurately quantifying ADAMTS5 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its user-friendly design and exceptional performance, the Human ADAMTS5 ELISA Kit is an essential tool for researchers studying ADAMTS5 and its role in disease pathology.

Product Name:Human A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit
Product Code:HUDL00068
Size:96 Assays
Target:ADAMTS5
Synonyms:ADMP-2, Aggrecanase-2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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