[KO Validated] HIF1AN/FIH1 Rabbit Polyclonal Antibody (CAB5466)
- SKU:
- CAB5466
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
Product Name: | [KO Validated] HIF1AN/FIH1 Rabbit Polyclonal Antibody |
SKU: | CAB5466 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2). |
Sequence: | MAAT AAEA VASG SGEP REEA GALG PAWD ESQL RSYS FPTR PIPR LSQS DPRA EELI ENEE PVVL TDTN LVYP ALKW DLEY LQEN IGNG DFSV YSAS THKF LYYD EKKM ANFQ NFKP RSNR EEMK FHEF VEKL QDIQ QRGG EERL YLQQ TLND TVGR KIVM DFLG FNWN WINK QQGK RGWG QLTS NLLL IGME GNVT PAHY DEQQ NFFA QIKG YKRC ILFP PDQF ECLY PYPV HHPC DRQS QVDF DNPD YERF PNFQ NVVG YETV VGPG DVLY IPMY WWHH IESL LNGG ITIT VNFW YKGA PTPK RIEY PLKA HQKV AIMR NIEK MLGE ALGN PQEV GPLL NTMI KGRY N |
Tested Applications: | WB IHC-P IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | FIH1; H1 |
Positive Sample: | MCF7,K-562,U-937,293T,Mouse heart,Mouse skeletal muscle |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus, perinuclear region. |
Calculated MW: | 41kDa |
Observed MW: | 40kDa |
Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.
Purification Method: | Affinity purification |
Gene ID: | 55662 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |