The Elongin B Polyclonal Antibody (CAB5362) is a valuable tool for researchers studying Elongin B, a protein complex involved in transcriptional regulation and ubiquitin-dependent proteolysis. This antibody, raised in rabbits, exhibits high reactivity with Elongin B in human samples and has been validated for use in Western blot applications.Elongin B is known to play a crucial role in the assembly of the Elongin BC complex, which functions as a substrate recognition module in the ubiquitin ligase complex. This complex is involved in targeting specific proteins for degradation, making Elongin B an important player in cellular processes such as gene expression and protein turnover.
By using the Elongin B Polyclonal Antibody, researchers can detect and analyze Elongin B in various cell types, providing insights into its function and mechanisms of action. This antibody is particularly useful for studies in molecular biology, biochemistry, and cancer research, where Elongin B has been implicated in tumorigenesis and potential therapeutic targets. Understanding the role of Elongin B in these contexts is essential for developing targeted therapies and furthering our knowledge of cellular processes.
Product Name:
TCEB2 Rabbit Polyclonal Antibody
SKU:
CAB5362
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human TCEB2 (NP_009039.1).
This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
Purification Method:
Affinity purification
Gene ID:
6923
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TCEB2 Rabbit pAb (CAB5362) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
