The DCLK2 Polyclonal Antibody (PAC021896) is a specialized tool for researchers studying DCLK2, a protein known for its role in cancer progression and metastasis. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it an excellent choice for Western blotting experiments. By binding specifically to the DCLK2 protein, this antibody allows for precise detection and analysis in a variety of cell types, offering valuable insights into cancer biology.DCLK2, a marker of tumor initiating cells, is implicated in promoting epithelial-to-mesenchymal transition, a critical process in cancer metastasis.
Its association with aggressive tumor behavior highlights its potential as a therapeutic target for combating cancer progression. By investigating the function of DCLK2, researchers can uncover new avenues for developing targeted anticancer therapies and improving patient outcomes.Overall, the DCLK2 Polyclonal Antibody (PAC021896) serves as a powerful tool for unraveling the mechanisms underlying cancer cell plasticity and metastatic spread, paving the way for innovative treatment strategies and personalized medicine approaches.
Antibody Name:
DCLK2 Antibody (PACO21896)
Antibody SKU:
PACO21896
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human DCLK2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells, using DCLK2 antibody.
Background:
Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm By similarity. Hillier L.W., Nature 434:724-731(2005).
DCAMKL2: Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Kinase, protein; Protein kinase, CAMK; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; DCAMKL family
Chromosomal Location of Human Ortholog: 4q31.3
Cellular Component: cytoskeleton; cytoplasm
Molecular Function:protein serine/threonine kinase activity; ATP binding
Biological Process: protein amino acid phosphorylation
NCBI Summary:
This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]
