The Caspase-3 Polyclonal Antibody (CAB11319) offered by Assay Genie is a valuable tool for researchers studying apoptosis and programmed cell death. Caspase-3 is a critical enzyme in the apoptosis pathway, playing a key role in cell death and survival processes. This antibody, generated in rabbits, is highly specific for caspase-3 and has been validated for use in Western blotting applications.This antibody binds specifically to caspase-3 protein, allowing for accurate detection and analysis in a variety of cell types. Its high reactivity with human samples makes it an ideal choice for researchers studying apoptosis in the context of diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
By using the Caspase-3 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying apoptosis and its dysregulation in various disease states. Understanding the role of caspase-3 in cell death pathways may lead to the development of novel therapeutic strategies targeting apoptosis for the treatment of cancer and other diseases.
Product Name:
Caspase-3 Rabbit Polyclonal Antibody
SKU:
CAB11319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 6-105 of human Caspase-3 (NP_004337.2).
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Caspase-3 Rabbit pAb (CAB11319) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
