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Bovine Pigment Epithelium Derived Factor (PEDF) ELISA Kit

SKU:
BODL00135
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q95121
Sensitivity:
0.135ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SERPINF1,EPC1,PIG35,SDF3,Serpin F1,Cell proliferation-inducing gene 35,Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin),Stromal Cell Derived Factor 3
Reactivity:
Bovine
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
Frequently bought together:

Description

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Bovine Pigment Epithelium Derived Factor (PEDF) ELISA Kit

The Bovine Pigment Epithelium-Derived Factor (PEDF) ELISA Kit is specifically designed for the precise detection of PEDF levels in bovine samples like serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PEDF is a vital protein that plays a key role in regulating angiogenesis, inhibiting tumor growth, and promoting neuronal survival.

It is implicated in various diseases such as age-related macular degeneration, diabetic retinopathy, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Bovine PEDF ELISA Kit is an essential tool for researchers looking to investigate the role of PEDF in various physiological and pathological processes in bovine models.

Product Name:Bovine Pigment Epithelium Derived Factor (PEDF) ELISA Kit
Product Code:BODL00135
Size:96 Assays
Target:PEDF
Synonyms:SERPINF1, EPC1, PIG35, SDF3, Serpin F1, Cell proliferation-inducing gene 35, Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin), Stromal Cell Derived Factor 3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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