Whitepapers

The MycoGenie Rapid Mycoplasma Detection Kit enables the detection of 28 Mycoplasma species in under 1 hour using a simple visual determination method, eliminating the need for expensive equipment and complex protocols. The kit demonstrated a detection limit of 3.07 × 10² Mycoplasma copies, matching the sensitivity of the "Gold Standard" qPCR method. The assay utilizes an isothermal amplification method with a colorimetric readout, allowing easy, rapid, and reliable detection without specialized training. The MycoGenie kit is compatible with a wide range of cell types and culture media, making it a versatile solution for cell culture laboratories. Mycoplasma contamination was s …

The 3D Organoid Cell Viability Assay (AKES081) demonstrated high sensitivity and low cytotoxicity, making it ideal for long incubation studies (24-48 hours) and high-throughput applications. AKES081 effectively quantified live cell numbers in human colon organoids using WST-8 reduction, providing a direct correlation between viability and optical density at 450 nm. AKES081 confirmed expected cytotoxic effects of 5-fluorouracil (5-FU), with decreasing organoid survival at increasing drug concentrations, validating its suitability for drug screening applications. The one-step, wash-free protocol enabled easy viability assessment and allowed for subsequent RNA extraction for gene …

MORV0013 functioned effectively in both qPCR machines and water baths, with or without paraffin oil, maintaining consistent sensitivity and specificity. MORV0013 performed reliably between 60°C and 67°C, ensuring accurate mycoplasma detection even in instruments with varying temperature precision. MORV0013 maintained specificity and accuracy over extended reaction times (2 hours), demonstrating robustness for prolonged testing conditions. MORV0013 successfully detected mycoplasma in multiple cell culture types (suspension and adherent, human and mouse), confirming broad applicability. Different culture media and serum types had no significant effect on amplification speed, sens …

The Assay Genie 3D Organoid Cell Viability Assay (AKES081) provided superior results compared to ThermoScientific PrestoBlue, maintaining over 100% viability at all time points in untreated 3D apical-out airway organoids. AKES081 detected reduced cell viability (0.5% to 5.9%) when organoids were treated with 10% DMSO, demonstrating its ability to accurately measure cytotoxic effects. AKES081 maintained consistent and reliable readings across 1-hour, 3-hour, and overnight incubations, making it suitable for high-throughput and long-term studies. Unlike resazurin-based methods, the WST-8-based assay provided more stable results with minimal interference, allowing for extended inc …

Both Assay Genie MORV0010 and Promega E1910 effectively quenched Firefly luciferase before Renilla detection, minimizing signal overlap. E1910 achieved 99.95% quenching efficiency, while MORV0010 achieved 99.90%, ensuring accurate dual luciferase measurements. MORV0010 maintained low background luminescence (680 RLU) comparable to E1910 (639 RLU), ensuring sensitive Renilla luciferase detection without interference. MORV0010 exhibited slightly higher initial Firefly luminescence values, offering robust signal strength for detecting gene expression changes. Despite its economical pricing, the Assay Genie Dual Luciferase Reporter Assay Kit (MORV0010 ) delivers results comparable …

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly the negative impact of +4°C (fridge) storage over time, confirming its sensitivity to environmental stress. AKES081 successfully differentiates viability effects based on temperature and duration, while flask sealing (O₂ concentration) shows no significant influence. Raw absorbance values and ANOVA analysis confirm AKES081 ability to detect statistically significant differences between control and test conditions. AKES081 provides a reliable tool for evaluating viability in live BENO delivery, b …

The Dynex DS2 platform fully automates the MR-proANP and MR-proADM ELISA assays, streamlining sample processing from loading to detection. Automation ensures consistent assay performance with a coefficient of variation (CV) below 10%, minimizing variability across runs. The system enables high-throughput analysis of multiple 96-well plates, making it ideal for large-scale biomarker research. Hands-on time is significantly reduced, allowing researchers to focus on other tasks without compromising data quality. The automated workflow maintains assay sensitivity and linearity, ensuring accurate biomarker quantification for cardiovascular research applications.Automation of MR-proA …