Blog

RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein from cultured cells. RIPA buffer cell lysis enables determination of protein concentration. RIPA buffer is an ideal cell lysis reagent since it contains three non-ionic and ionic detergents. Preserving protein:protein interactions When you need to preserve protein-protein interactions or to reduce denaturation its recommended to use a RIPA buffer recipe without SDS (ionic detergent) or Triton X-100 (non-ionic detergent). RIPA buffer recipes …

We know it gets tiring being in the lab every day, especially with the evenings getting darker and gloomier. So, to get you in the festive spirit, we're holding a competition to win money to throw a Christmas lab party! We have two prizes - one of €300 and another of €150. All you have to do to enter is write us an 800 word blog post on one of the following topics: Cell metabolism Immunology Signalling Cancer research The submissions will be posted on our site with credit. Some examples of previous submissions can be found here: Auto-inflammatory diseases and genetics Chemokines and Chemokine Receptors NLR inflammasomes, the enigmatic drivers of Inflammation Entries can be sent to …