Blog
Time-Resolved Fluorescence (TRF): A Comprehensive Introduction
Time-Resolved Fluorescence (TRF) technology is a cutting-edge analytical technique that has revolutionized the fields of biochemistry, immunology, and molecular biology. By offering a refined appr
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14th Mar 2024
Understanding Blotchy Western Blots: Causes and Remedies
Western blotting is a powerful and widely used technique in molecular biology, essential for detecting and quantifying specific proteins in complex biological samples. However, despite its popu
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13th Mar 2024
Control Samples Required for ELISA Protocol
Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful analytical biochemistry assay that utilizes antibodies and color change to identify a substance. It is widely used in the field of immunolog
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2nd Mar 2024
Fluorescence Resonance Energy Transfer (FRET) Assays: An Insight into Molecular Interactions
Fluorescence Resonance Energy Transfer (FRET) assays represent a pivotal technique in the molecular and cellular biology fields, enabling the examination of protein interactions, nucleic acid stru
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1st Mar 2024
Unveiling the Potential of Cy3 Wavelength: Illuminating the Path to Advanced Biomedical Imaging
In the realm of biomedical imaging, the quest for precision and clarity drives researchers to explore innovative technologies. One such breakthrough is the Cy3 wavelength, a powerful tool revol
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23rd Feb 2024
Choosing a Fluorescent Protein: A Comprehensive Guide
Fluorescent proteins (FPs) have revolutionized the field of molecular and cellular biology, allowing scientists to visualize and track biological processes in live cells with unprecedented clarity
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17th Feb 2024
Navigating the Intricacies of Intracellular Flow Cytometry: Key Considerations
In the evolving landscape of cellular biology, intracellular flow cytometry (ICFC) stands out as a powerful technique for analyzing the complexities within cells. This advanced method extends the
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16th Feb 2024
Optimizing Organoid Culture Conditions: Paving the Way for Revolutionary Advances in Biomedical Research
In the dynamic landscape of biomedical research, organoids have emerged as a groundbreaking tool, offering three-dimensional (3D) models that mimic the complex architecture and functionality of hu
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14th Feb 2024
What is the difference between PBS and dPBS?
In the realm of biological and biochemical research, solutions play a pivotal role in various experimental procedures, from cell culture to molecular biology assays. Among the plethora of solut
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8th Feb 2024
Transduction vs Transfection: Understanding Gene Delivery Techniques
In the realm of molecular biology and genetic engineering, the ability to deliver genetic material into cells is fundamental for various research and therapeutic purposes. Two common methods em
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8th Feb 2024
Guide to Western Blot Sample Preparation
Western blotting remains a pivotal technique in the molecular biosciences for the detection, quantification, and analysis of proteins. Its utility spans numerous fields, including immunology, dev
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6th Feb 2024
Mouse Injection Guide (Subcutaneous, Instramuscular & Intraperitonael)
In scientific research involving mice, the administration of substances via injection is a common practice. Whether delivering drugs, antibodies, or experimental compounds, precise injection te
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4th Feb 2024
Serum vs. Plasma: A Deep Dive into Their Molecular Makeup and Implications for ELISA
Blood plasma and serum are essential components of blood and have distinct molecular compositions and uses in the medical and research fields. This article aims to elucidate the differences betwee
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24th Jan 2024
Hybridoma Technology: Revolutionizing Antibody Production
Hybridoma technology, a revolutionary method in immunology pioneered by Georges Köhler and César Milstein in the 1970s, has transformed the landscape of antibody production. This groun
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23rd Jan 2024
Exploring Organelle Markers: Illuminating the Cellular Landscape
The eukaryotic cell is a complex and dynamic system with distinct membrane-bound compartments known as organelles, each performing specialized functions essential for cellular survival. To better
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11th Jan 2024
In Vitro Vs In Vivo Studies
Discover the distinct roles of 'In Vivo' and 'In Vitro' in biological research, where one offers a natural context and the other precise control, both vital for scientific advancement.
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2nd Aug 2023
Transfection: A Comprehensive Guide
Transfection protocols play a crucial role in the realm of molecular biology and genetic research,
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12th Jul 2023
Transformation Protocol – Sample Transformation Protocols
Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact
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27th Jun 2023
Mycoplasma in Cell Culture Detection Guide
As a PhD student delving deep into the realm of cellular biology, there is one antagonist that consi
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28th May 2023
Renal and Hepatic Function Assays
Hepatic Function Assays
Liver function assays are a suite of tests designed to assess the health and efficacy of our vital
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7th Mar 2023
Flow Cytometry Protocol | 10 Hints & Tips
Flow Cytometry Protocol
The meaning is in the name:
Flow = in motion, Cyto = cells, Metry = measure.
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7th Nov 2022
Cell Synchronisation Methods
What is Cell Synchronisation?
Cell synchronisation is a process by which cells at different stages of the cell cycle in a cultu
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26th Oct 2022
Guide to Fluorescence Activated Cell Sorting (FACs): The Latest Technology in Cell Separation
Fluorescence activated cell sorting (FACS) is a cutting-edge technique used to isolate individual cells from a sample and analyse their properties. In this guide, we will discuss the basics
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26th Jul 2022
RIPA Buffer Recipe - Cell Lysis | Assay Genie
RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein fro
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20th Aug 2021