The ZNF224 Antibody (PACO59936) is a polyclonal antibody designed for research involving ZNF224, a zinc finger protein known to play a role in gene transcription and regulation. This antibody is raised in rabbits and has high reactivity with human samples, making it a valuable tool for studying the function of ZNF224 in various cellular processes.ZNF224 is involved in the control of gene expression and has been implicated in the regulation of cell growth and differentiation. By binding to the ZNF224 protein, this antibody allows for the detection and analysis of ZNF224 in different cell types, making it an essential tool for researchers in the fields of molecular biology and cancer research.
The role of ZNF224 in gene regulation and its potential involvement in cancer make it a promising target for further investigation. Understanding the function of ZNF224 could provide insights into the development of new therapies for cancer and other diseases where gene expression plays a critical role. The ZNF224 Antibody (PACO59936) is a valuable resource for researchers looking to explore the complex mechanisms of gene regulation and its implications in health and disease.
IHC image of PACO59936 diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO59936 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
Synonyms:
Zinc finger protein 224 (Bone marrow zinc finger 2) (BMZF-2) (Zinc finger protein 233) (Zinc finger protein 255) (Zinc finger protein 27) (Zinc finger protein KOX22), ZNF224, BMZF2 KOX22 ZNF233 ZNF255 ZNF27
UniProt Protein Function:
ZNF224: a probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family.Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger proteinChromosomal Location of Human Ortholog: 19q13.2Cellular Component: nuclear membrane; nucleus; transcriptional repressor complexMolecular Function: protein binding; transcription factor activityBiological Process: negative regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein represses transcription of the aldolase A gene, which encodes a key enzyme in glycolysis. The encoded zinc-finger protein may also function as a transcriptional co-activator with Wilms' tumor protein 1 to regulate apoptotic genes in leukemia. [provided by RefSeq, Jul 2016]
