The VPS18 Polyclonal Antibody (CAB16654) is specifically designed for research involving VPS18, a key protein involved in the endosomal sorting complex required for transport (ESCRT) pathway. This rabbit polyclonal antibody is highly reactive with human samples and is validated for use in Western blot applications. It binds to the VPS18 protein, allowing for the detection and analysis of VPS18 in various cell types, making it ideal for studies in cell biology and vesicle trafficking research.VPS18 is a crucial component of the ESCRT pathway, which is involved in the sorting and trafficking of proteins within the cell.
Dysregulation of this pathway has been linked to various diseases, including cancer, neurodegenerative disorders, and viral infections. Understanding the function of VPS18 is essential for unraveling the mechanisms underlying these diseases and developing targeted therapies.The VPS18 Polyclonal Antibody (CAB16654) offers a valuable tool for researchers studying vesicle trafficking, protein sorting, and cellular transport processes. Its high reactivity and specificity make it a reliable choice for investigating the role of VPS18 in various cellular processes and disease states.
Product Name:
VPS18 Polyclonal Antibody
SKU:
CAB16654
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide of human VPS18
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
PEP3; VPS18
Positive Sample:
A-549,mouse brain,rat brain
Conjugate:
Unconjugated
Cellular Localization:
autophagosome, clathrin-coated vesicle, CORVET complex, early endosome, endosome, glutamatergic synapse, late endosome, lysosome, presynapse
Calculated MW:
110kDa
Observed MW:
110kDa
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Purification Method:
Affinity purification
Gene ID:
57617
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS18 Rabbit pAb (CAB16654) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 4min.
