The TRMT2A Polyclonal Antibody (CAB7095) is a key tool for researchers studying TRMT2A, a protein involved in tRNA modification and mRNA translation. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications. By targeting the TRMT2A protein, researchers can effectively detect and analyze its expression levels in various cell types, facilitating studies in molecular biology and cancer research.
TRMT2A is essential for maintaining proper protein synthesis and gene regulation, making it a crucial player in cellular function and development. Its role in tRNA modification also suggests potential implications in disease, particularly in cancer and other genetic disorders. By unraveling the functions of TRMT2A, researchers can gain insights into the molecular mechanisms underlying disease pathology and potentially identify novel therapeutic targets for intervention.
Antibody Name:
Anti-TRMT2A Antibody
Antibody SKU:
CAB7095
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC
Reactivity:
Human
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2A (NP_073564.3).
Application:
WB IHC
Recommended Dilution:
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:
Human
Positive Samples:
HL-60, Raji, NCI-H460
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2A (NP_073564.3).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:
HTF9C: May be involved in nucleic acid metabolism and/or modifications. 2 isoforms of the human protein are produced by alternative splicing.
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
tRNA methyltransferase 2 homolog A (S. cerevisiae)
NCBI Official Symbol:
TRMT2AÂ Â
NCBI Official Synonym Symbols:
HTF9CÂ Â
NCBI Protein Information:
tRNA (uracil-5-)-methyltransferase homolog A; tRNA (uracil-5-)-methyltransferase homolog A; hpaII tiny fragments locus 9c protein; TRM2 tRNA methyltransferase 2 homolog A
UniProt Protein Name:
tRNA (uracil-5-)-methyltransferase homolog A
UniProt Synonym Protein Names:
HpaII tiny fragments locus 9c protein
Protein Family:
tRNA
UniProt Gene Name:
TRMT2AÂ Â
UniProt Entry Name:
TRM2A_HUMAN
Western blot analysis of extracts of various cell lines, using TRMT2A antibody (CAB7095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using TRMT2A antibody (CAB7095) at dilution of 1:100 (40x lens).
