The TRIM63 Polyclonal Antibody (CAB3101) is a reliable tool for researchers studying the function of TRIM63, a protein involved in skeletal muscle atrophy and regulation of muscle mass. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in various applications such as Western blotting. It binds specifically to the TRIM63 protein, allowing for accurate detection and analysis in different cell types.TRIM63, also known as MuRF1, is a crucial player in the process of muscle degradation and has been linked to various muscle-related diseases and conditions.
Its role in the regulation of muscle mass makes it a valuable target for research in muscle physiology, metabolism, and diseases like cachexia and muscle wasting disorders. Understanding the function of TRIM63 is essential for developing interventions and therapies aimed at preserving muscle mass and improving overall health and wellbeing.
Product Name:
TRIM63 Rabbit Polyclonal Antibody
SKU:
CAB3101
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 253-353 of human TRIM63 (NP_115977.2).
Cytoplasm, M line, Nucleus, Z line, myofibril, sarcomere.
Calculated MW:
40kDa
Observed MW:
47kDa
This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.
Purification Method:
Affinity purification
Gene ID:
84676
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of 293F, using TRIM63 antibody (CAB3101) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
