The TP53BP1 Polyclonal Antibody (CAB5757) is a valuable tool for researchers studying TP53BP1, a key player in DNA damage response and repair pathways. This antibody, produced in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications. By targeting the TP53BP1 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in oncology and genetic research.TP53BP1, also known as tumor protein p53 binding protein 1, is a crucial regulator of the cellular response to DNA damage, playing a vital role in maintaining genomic stability and preventing tumorigenesis. Understanding the function and regulation of TP53BP1 is essential for unraveling the mechanisms underlying cancer development and identifying potential therapeutic targets for cancer treatment.
Researchers exploring the intricate pathways involved in DNA repair and cell cycle control will benefit from the TP53BP1 Polyclonal Antibody (CAB5757), as it provides a reliable tool for studying the function and expression of TP53BP1 in various experimental settings. Its application in Western blot analysis allows for accurate quantification and localization of TP53BP1, facilitating in-depth investigations into the molecular mechanisms governing DNA damage response pathways.
Product Name:
53BP1 Rabbit Polyclonal Antibody
SKU:
CAB5757
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 895-1031 of human TP53BP1 (NP_005648.1).
Sequence:
FCES SSET PFHF TLPK EGDI IPPL TGAT PPLI GHLK LEPK RHST PIGI SNYP ESTI ATSD VMSE SMVE THDP ILGS GKGD SGAA PDVD DKLC LRMK LVSP ETEA SEES LQFN LEKP ATGE RKNG STAV AESV ASPQ K
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
p202; 53BP1; TDRD30; p53BP1
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, centromere, kinetochore.
Calculated MW:
214kDa
Observed MW:
450kDa
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
7158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using 53BP1 Rabbit pAb (CAB5757) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
