The Thioredoxin Polyclonal Antibody (CAB7638) is a valuable tool for researchers studying Thioredoxin, a protein that plays a key role in redox regulation and cell signaling pathways. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to Thioredoxin, this antibody enables accurate detection and analysis of the protein in various cell types.Thioredoxin is essential for maintaining cellular redox balance and has been implicated in a wide range of physiological processes, including cell growth, apoptosis, and immune responses.
Dysregulation of Thioredoxin has been linked to various diseases, such as cancer, neurodegenerative disorders, and cardiovascular diseases. By studying the function and regulation of Thioredoxin, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Thioredoxin Polyclonal Antibody (CAB7638) offers researchers a reliable tool for investigating the role of Thioredoxin in health and disease, making it a valuable asset for studies in molecular biology, biochemistry, and biomedicine.
WB,1:500 - 1:2000 IHC-P,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TRX; TRDX; TRX1; Trx80; Thioredoxin 1 (Trx1/TXN)
Positive Sample:
HepG2,H460,MCF7,HT-29,SKOV3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, Secreted.
Calculated MW:
12kDa
Observed MW:
12kDa
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Thioredoxin 1 (Trx1/TXN) Rabbit pAb (CAB7638) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
