The SMPD4 Polyclonal Antibody (CAB15473) is a valuable tool for researchers studying sphingomyelin phosphodiesterase 4 (SMPD4), an enzyme involved in the regulation of sphingolipid metabolism, cell signaling, and cell death processes. The antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blot applications.SMPD4 is known to play a crucial role in cellular processes such as apoptosis, cell growth, and differentiation, making it a potential therapeutic target for various diseases, including cancer and neurodegenerative disorders. By detecting and analyzing SMPD4 protein levels in different cell types, this antibody enables researchers to investigate the function and regulation of SMPD4 in various biological contexts.
The SMPD4 Polyclonal Antibody is a valuable tool for researchers interested in understanding the molecular mechanisms underlying cell death pathways and lipid metabolism, as well as exploring the potential therapeutic applications of targeting SMPD4 in human diseases. Its high specificity and sensitivity in detecting SMPD4 make it an ideal choice for studies in the fields of cell biology, biochemistry, and drug discovery.
Product Name:
SMPD4 Rabbit Polyclonal Antibody
SKU:
CAB15473
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2).
The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network.
Purification Method:
Affinity purification
Gene ID:
55627
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using SMPD4 Rabbit pAb (CAB15473) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
