The SLC22A25 Polyclonal Antibody (PACO55962) is a valuable tool for researchers studying the SLC22A25 protein, which belongs to the solute carrier family of membrane transport proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By targeting the SLC22A25 protein, this antibody enables researchers to detect and analyze the expression of SLC22A25 in various cell types, providing insights into its role in cellular transport and metabolism.SLC22A25 is known to play a crucial role in the transport of endogenous compounds, drugs, and toxins across cell membranes, making it a key player in drug metabolism and detoxification processes.
Research on SLC22A25 is essential for understanding the mechanisms of drug transport and metabolism, which has implications for drug development and personalized medicine. The SLC22A25 Polyclonal Antibody is a valuable tool for researchers interested in unraveling the functions of SLC22A25 in health and disease, particularly in the fields of pharmacology and toxicology.
Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: SLC22A25 antibody at 6.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa.
Immunofluorescence staining of HepG2 cells with PACO55962 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO55962 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
integral component of plasma membrane, inorganic anion exchanger activity, sodium-independent organic anion transmembrane transporter activity, urate transmembrane transporter activity, sodium-independent organic anion transport, urate transport
Synonyms:
Solute carrier family 22 member 25 (Organic anion transporter UST6), SLC22A25, UST6
