The SEPHS1 Polyclonal Antibody (CAB6454) is specifically designed for research involving SEPHS1, a crucial enzyme in selenocysteine biosynthesis. This antibody, produced in rabbits, is highly reactive with SEPHS1 in human samples and has been validated for use in Western blot applications. By binding to the SEPHS1 protein, this antibody enables the detection and analysis of SEPHS1 in various cell types, making it ideal for studies in biochemistry and molecular biology.SEPHS1 plays a critical role in maintaining cellular selenium homeostasis and is essential for the biosynthesis of selenoproteins, which are important for antioxidant defense mechanisms and overall cellular health.
Research into SEPHS1 is crucial for understanding the impact of selenium deficiency on human health and for developing strategies to improve selenium utilization in the body.By utilizing the SEPHS1 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms of selenocysteine biosynthesis and the regulation of selenoprotein expression. This antibody is a valuable tool for studies investigating the role of selenium in human health, including its potential as a therapeutic target for conditions related to selenium deficiency.
Product Name:
SEPHS1 Rabbit Polyclonal Antibody
SKU:
CAB6454
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
Purification Method:
Affinity purification
Gene ID:
22929
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SEPHS1 Rabbit pAb (CAB6454) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
