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RPA70 Rabbit Polyclonal Antibody (CAB9841)

SKU:
CAB9841
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 3 Anti-RPE65 Antibody CAB9841
  • Signal Transduction Antibodies 3 Anti-RPE65 Antibody CAB9841
  • Signal Transduction Antibodies 3 Anti-RPE65 Antibody CAB9841
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:RPA70 Rabbit Polyclonal Antibody
SKU:CAB9841
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 274-533 of human RPE65 (NP_000320.1).
Sequence:NYMD CFES NETM GVWL HIAD KKRK KYLN NKYR TSPF NLFH HINT YEDN GFLI VDLC CWKG FEFV YNYL YLAN LREN WEEV KKNA RKAP QPEV RRYV LPLN IDKA DTGK NLVT LPNT TATA ILCS DETI WLEP EVLF SGPR QAFE FPQI NYQK YCGK PYTY AYGL GLNH FVPD RLCK LNVK TKET WVWQ EPDS YPSE PIFV SHPD ALEE DDGV VLSV VVSP GAGQ KPAY LLIL NAKD LSEV ARAE VEIN IPVT FHGL FKKS
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:200
Synonyms:p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; RPE65
Positive Sample:Mouse eye
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Lipid-anchor, Microsome membrane.
Calculated MW:61kDa
Observed MW:65kDa

The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital.

Purification Method:Affinity purification
Gene ID:6121
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (CAB9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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