Recombinant Rat IL-1Ra/IL-1F3/IL-1RN Protein (RPCB1823)

Product Name:	Recombinant Rat IL-1Ra/IL-1F3/IL-1RN Protein
SKU:	RPCB1823
Size:	10µg
Tag:	NO-tag
Reactivity:	Rat
Expression Host:	E. coli

Protein Description:	High quality, high purity and low endotoxin recombinant Recombinant Rat IL-1Ra/IL-1F3/IL-1RN Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:	< 1EU/μg
Purity:	> 92% by SDS-PAGE.
Formulation:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:	60582

Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A), and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein-encoding gene is reported to be associated with an increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.

Storage:	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:	Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:	P25086

	Recombinant Rat IL-1Ra/IL-1F3/IL-1RN Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25 kDa.
	Recombinant Rat IL-1Ra/IL-1F3/IL-1RN inhibit IL-1 alpha-dependent proliferation in D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 2.62-10.48 ng/mL in the presence of 50 pg/mL of rrIL-1 alpha, corresponding to a specific activity of 9.54×104~3.82×105 units/mg.